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KMID : 0620920210530101547
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Experimental & Molecular Medicine
2021 Volume.53 No. 10 p.1547 ~ p.1558
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circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3
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Huang Yizhen
Zhang Zhenlei
Wang Jianle
Shen Shuying
Yao Teng
Xu Yining
Chen Zizheng
Fang Bin
Ma Jianjun
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Abstract
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The abnormal expression of circular RNAs (circRNAs) is associated with numerous human diseases. This study investigated the mechanism by which circRNA acts as competitive endogenous RNA in the regulation of degenerative intervertebral disc disease (IVDD). Decreased expression of circSPG21 was detected in degenerated nucleus pulposus cells (NPCs), the function of circSPG21 in NPCs was explored and verified, and the downstream target of circSPG21 was investigated. The interaction between circSPG21 and miR-1197 and its target gene (ATP1B3) was studied by online database prediction and molecular biological verification. Finally, the circSPG21/miR-1197/ATP1B3 axis was verified in the mouse tail-looping model. The expression of circSPG21 in the nucleus pulposus in IVDD was directly related to an imbalance of anabolic and catabolic factors, which affected cell senescence. circSPG21 was found to play a role in human NPCs by acting as a sponge of miR-1197 and thereby affecting ATP1B3. The regulation of circSPG21 provides a potentially effective therapeutic strategy for IVDD.
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KEYWORD
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Long non-coding RNAs, Mechanisms of disease
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